Recombinant Fc gamma RII (CD32) 抗体
Quick Overview for Recombinant Fc gamma RII (CD32) 抗体 (ABIN7795371)
抗原
See all Fc gamma RII (CD32) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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Expression System
- Mammalian cells
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原理
- In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3)
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产品特性
- The recombinant IV.3 antibody binds to human CD32 (FcγRII or FCGR2), a cluster of differentiation molecule found on the surface of a variety of immune cells. CD32 is a surface receptor glycoprotein belonging to the Ig gene superfamily, has a low-affinity for the Fc region of IgG antibodies in monomeric form, but high affinity for IgG immune complexes. CD32 has two major functions: cellular response regulation, and the uptake of immune complexes. CD32 consists of Fc receptors FcγRIIa (CD32a), FcγRIIb (CD32b), and FcγRIIc (CD32c). The most popular human Fc blocking reagents include: Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining, it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity. Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls. Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking. Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. Best choice of human Fc blocking reagents based on your application are: For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22). For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable, the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins. For Western Blot and ELISA: BSA or FBS. For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.
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纯化方法
- Protein A or G affinity column
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纯度
- >95 % by reducing SDS-PAGE
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内毒素水平
- < 1 EU per 1 mg of the protein by the LAL method
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亚型
- Fc Silenced, IgG1
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anti-Fc gamma RII (CD32) antibody
CD32 适用: 人 FACS, WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6D11 unconjugated
anti-Fc gamma RII (CD32) (AA 201-300) antibodyCD32 适用: 人 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 PE
anti-Fc gamma RII (CD32) (AA 69-309) antibodyCD32 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibody (PE)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 PE
anti-Fc gamma RII (CD32) antibody (FITC)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 FITC
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人, 犬, 猕猴, Pig FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal AT10 unconjugated
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: Cow, 绵羊 FACS 宿主: 小鼠 Monoclonal CCG36 PE
anti-Fc gamma RII (CD32) antibody (FITC)CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 FITC
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- > 3 mg/mL
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储存条件
- 4 °C,-20 °C
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储存方法
- Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.
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- Fc gamma RII (CD32)
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别名
- CD32
抗原
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